What is phage display technology?

Phage display technology is to insert the DNA sequence of the foreign protein or polypeptide into the appropriate position of the phage coat protein structural gene, so that the foreign gene is expressed along with the expression of the coat protein. At the same time, the foreign protein is renewed with the phage.

Principles of phage display technology

Phage display technology is to clone the polypeptide or protein coding gene or target gene fragment into the appropriate position of the phage coat protein structural gene. Under the condition that the reading frame is correct and does not affect the normal function of other coat proteins, the exogenous polypeptide or protein can be combined with the coat. The protein is expressed in fusion, and the fusion protein is displayed on the surface of the phage as the progeny phage is reassembled. The displayed polypeptide or protein can maintain a relatively independent spatial structure and biological activity to facilitate the recognition and binding of the target molecule.

Over Solutions

Using phage display technology to discover fully human therapeutic monoclonal antibodies

• CD BioSciences has a fully human phage library with a diversity of up to 10^11 to ensure the effectiveness of screening. At the same time, automated instruments used in high-throughput experiments accelerate these screening processes.
• All variable region genes are amplified with high fidelity from different healthy people, and strict cloning procedures ensure a high proportion of functional clones in the library.
• The VH and VL libraries are independent of each other, thereby maximizing the acquisition of V genes, and also providing convenience for subsequent affinity maturation based on chain replacement.
• All V gene families are cloned and assembled independently to maximize the diversity of the library and reduce the gene bias of PCR cloning.
• The first natural antibody library is constructed based on the preset V gene combination to match the human immune system

Affinity maturation (based on phage display and high-throughput screening platform)

• Based on the optimization of the binding kinetics (dissociation rate), the Kd value can be increased to below nM level, or even to pM level
• Both scFv and Fab formats can be used to construct CDR mutation libraries.
• Using biotinylated antigen for liquid library panning, the concentration of the antigen can be lower.
• We use the off-rate screening method to screen pM-level binding molecules, and use non-labeled antigens for competitive binding.
• The enriched clones are amplified in 96-well plates, and high-throughput automated screening equipment is used to rank the affinity of different clones.
• The best clones that have been identified can be converted into full-length IgG and subjected to further Biacore and biological and functional analysis.

Comprehensive optimization of candidate monoclonal antibody properties 

• Germline of antibody genes to reduce immunogenicity
• Drug stability (temperature, chemical and proteolytic stability)
• Expression level, solubility, production feasibility
• IgG type conversion, Fc segment modification, antibody fragmentation, protein fusion, skeletonization

Our Services

 Our Advantages

• CD BioSciences has an experienced antibody humanization team whose technical expertise has contributed to the successful development and marketing of therapeutic antibody drugs.
• CD BioSciences has self-developed high expression vectors and cell lines with commercial development licenses.
• CD BioSciences can complete the entire therapeutic development from cell line development, optimization of fermentation and culture conditions, to fermentation in 150L cell tanks.

Why Choose Us?

CD BioSciences' phage display service is a personalized and customized innovative scientific research service. Each project needs to be evaluated before the corresponding analysis plan and price can be determined. If you want to know more about service prices or technical details, please feel free to contact us.